00:01
All right, so in this question, we're considering carbon monoxide and cyanide poisoning, essentially cytochrome c oxidase inhibition.
00:10
Now, we're asked to look what will be the effects in different parts of mitochondria, the ph of the intermembrane space, a concentration of nadh, simply inside, and then rate of production of atp in the matrix.
00:25
So let's, i guess, draw out a little mitochondria.
00:31
Boom, boom.
00:32
Here's a, we'll call this the outer membrane.
00:35
Let's use a highlighter for the inner membrane just so that we can draw on that.
00:40
There's the inner membrane and this will be the inter membrane space.
00:47
So let's go ahead and just draw the complexes in, right? that would be helpful.
00:53
So we've got complex one kind of looks like that.
00:56
We've got two kind of looks like that.
01:00
Q, you know, is it just closely related there because it's used.
01:05
We've got number three, probably going to run out of room here, number four, and then atp synthase.
01:14
So let's just extend that a bit.
01:17
It kind of looks like a wheel or something, and you've got those little pieces down here on the bottom.
01:24
Okay, so kind of the whole point of this electron transport chain that we've drawn out is that we have hydrogen ions or protons going in through here.
01:36
Okay, these are coming off of products from citrogatic cycle.
01:41
So, nadh is actually used in compound one or a complex one.
01:47
And you end up giving away that proton as well as an electron.
01:52
Okay, let's follow the electrons with a different color.
01:54
Let's use blue as well as an electron there.
01:58
And we end up with nad plus.
02:03
Kind of in complex 2, you have a similar thing going on with the fath, and we just end up with fad plus.
02:16
Kind of same idea.
02:18
We're going to have to track these electrons also.
02:22
But part of what powers this whole thing, all this draw comes from a reaction that's happening at the end that involves oxygen, right? that's what we're looking at.
02:32
This complex number four right there, this is actually it's called cytocrine c oxidase.
02:39
That's the name of the complex.
02:40
That's the same as four.
02:42
So if that was the issue, that's solved now.
02:45
We've also got cidrome c right here.
02:48
But let's just follow the pathways.
02:50
We're making lots and lots of hydrogen, ions as we're going on here.
02:58
The idea is that we're the mitochondria not asset necessarily is trying to build this proton gradients, where we have high proton concentration here and low over here, right? that powers in the end this atp synthase.
03:20
We sent through those protons and we output a ton of atp.
03:28
That's the goal of electron transport chain...