Assume that the reaction catalyzed by an enzyme follows Michaelis-Menten kinetics. The substrate concentration (Km, Michaelis constant) needed to reach 50% of the maximum reaction velocity (Vmax) is 25 ?M. What substrate concentration is required to obtain at least 95% of the maximum reaction velocity? 25 ?M 50 ?M 100 ?M 250 ?M 475 ?M
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We know that when [S] = Km, V = 0.5Vmax. In this case, Km = 25 pM. We want to find the substrate concentration [S] that gives V = 0.95Vmax. Show more…
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