Assume the amino acid sequence provided below is for amylase...

Assume the amino acid sequence provided below is for amylase, the enzyme that catalyzes the hydrolysis of starch into sugars. [7 marks] 5' - Met - Pro - Arg - Lys - Ala - Phe - STOP - 3' a) What sort of changes in the nucleotides can cause a frameshift mutation? Describe the impact of a frameshift mutation on the amino acid strand. [/3] b) Given the amino acid sequence above, create a possible frameshift mutation. Show the DNA coding and template strands, the mRNA strand and the resulting polypeptide chain. Underline & bold where you have inserted your mutation. [/4]

Transcript

00:01 Hello students, in this question we have been given an amino acid sequence of an enzyme as such and we have to find the mrna sequence, the cause of frame shift mutation and the dna coding strand and template strand.

00:28 So let's start with the process.

00:34 So now this is the amino acid sequence.

00:37 So we will be getting a 5 prime 3 prime mrna strand directly from it.

00:44 So metheonine is coded by a ug.

00:48 Proline can be cc n and arginine can be represented by cg n.

01:01 N is basically any of the four nucleotides.

01:06 Methionine proline arginine done lies is represented by either aaa or g, a .g.

01:18 Aline is represented by g, c, n.

01:24 And phenylalanine is represented by uu or c.

01:31 And stop cordon can be uag or uga or uaa.

01:38 Or uaaa so any of these three is possible so we do not at this moment consider which of the stop codon is present it can be any and it will not affect the structure but if i add say an amino acid or a nitrogen is base in between codon anywhere in between any of the code on it will change the sequence and it will affect the amino acid that is getting attached into the chain so this is how we can induce a frame shift mutation so if we have to take the if this is the mrna sequence so the complementary sequence would be the coding sequence or this we get the mrna sequence as a -u -g, c -c -n, c -g -g -n, a -a -a -a, let's take it as a, g -c -n, uuu -u, and ua -g.

03:11 So now this is my mrna sequence...

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