* Assume the magnification of the ocular is 10X.
2. What is working distance?
3. What is the total magnification of a microscope, using a 40X objective?
4. What is the total magnification of a microscope, using a 10X objective?
5. What is the field of view size using a 4X scanning objective?
6. What is the brightness of field using a 40X high objective?
7. State if the sentence below is True or False. Explain your reasoning.
"While focusing, starting with the lowest power is best."
8. Explain the difference between fine and coarse adjustment knobs. Which knob should be used at low magnification? Which knob should be used at high magnification?
9. Explain why beginners often "lose" their specimen of interest while shifting magnification from low to high power.
10. If you do not seem to find your specimen of interest at high magnification, what should be your course of action? List the various steps.
11. Based on your pre-lab reading, define the range of light microscope (in microns), range of electron microscope (in microns). Give two examples of organisms/specimens that you can observe using these two microscopes.
12. List three factors that determine the quality of a microscope image. Explain briefly how they play a role in image resolution. What parts of a microscope should one use to adjust these factors?