AZO to TIA flights Translation in prokaryotes vs euk. My Course - Genetics - M arning.com/ihub/assessment/f188d950-dd73-11e0-9572-0800200c9a66/71e4142c-6333-4af2-99c2-b48c t Lenovo McAfee Gmail YouTube Payroll Emplo Axolotl Question 8 of 10 What occurs during translation termination? The signal sequence binds to proteins in the ER membrane, so it can pass through the lipid bilayer. Through ATP, tRNA synthetases charge tRNAs in a two-step reaction. Initiation factors assist assembly of the ribosome at the translation start site. Release factors bind the stop codon and release the polypeptide chain from the tRNA in the P site. Search
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Which line below describes the termination of transcription? The termination of transcription occurs when the release factor sits on the nascent RNA formed when the termination sequence is reached. The sigma unit finds the promoter (TATA box) and calls RNA polymerase. Start codon reading is brought by mRNA. Which of the following describes codon usage? Codon usage refers to the specific codons used for each amino acid. All living things follow the genetic code. Each of the following molecules binds to the amino acid except: DNA The ribosome attaches to the mRNA at the P site in prokaryotes. The promoter region of a gene consists of consecutive nucleotides in DNA. In eukaryotes, the ribosome attaches to the mRNA at the 5' cap. The genetic code is universal, meaning that each amino acid is encoded by only one codon. The genetic code is degenerate, meaning that some amino acids have more than one codon. The genetic code is non-overlapping, meaning that each codon is read separately. The genetic code is commaless, meaning that there are no gaps between codons. The genetic code is unambiguous, meaning that each codon specifies only one amino acid. The genetic code is redundant, meaning that multiple codons can code for the same amino acid.
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5) How are the stop codons recognized at the end of translation process? Please describe the roles of release factors (RFs) in termination. Please also describe how the Ribosome Recycling Factor (RRF) operates for the dissociation of the mRNA and subunits of the ribosome at the end of translation. (10 points) 6) Please describe the function of the Shine-Dalgarno sequence in prokaryotic translation initiation. (10 points) 7) During translation elongation in procaryotes, the interplay between EF-Tu and EF-Ts regulate tRNA charging to the A site of the ribosome. Please describe in detail the sequence of events that take place during tRNA·EF-Tu·GTP ternary complex formation. Explain how the EF-Tu·GDP that is discharged from the ribosome is converted to EF-Tu·GTP that is the competent complex for translation elongation? What is the role of GTP hydrolysis (GTP bound to EF-Tu) in translation elongation? (15 points) 8) Please explain how the translation of Gcn4 is regulated by multiple open reading frames (ORFs) at the 5’-untranslated region of the transcript? What is the stimuli for the upregulation of Gcn4p translation? (15 points)
17. Fig. 12.21. Termination. How does the ribosome "know" when to stop translation? When the ribosome reaches a stop codon, such as ________, a release factor (rather than a tRNA) enters the A site. This release factor frees the polypeptide. In turn, the mRNA is released and the large and small ribosomal subunits dissociate.
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