B4. Indicate which one of the statements below is incorrect: a. In the case of the Illumina sequencing technology, the identification of the precise array of nucleotides in the fragment of DNA to be read is coupled with the synthesis of a new strand. b. The approach "overlap-layout-consensus" can be used during the assembly of a genome sequenced using Illumina. c. In Illumina sequencing, millions of fragments are read simultaneously, decreasing the time required to sequence any genome compared to Sanger sequencing. d. Both Sanger and Illumina sequencing use modified nucleotides that carry a fluorophore and a blocking group that prevents the addition of further nucleotides. The difference between both sequencing methods is that the nucleotide modification is reversible in the case of Illumina.
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Which of the following statements are correct? Explain your answers. A. Restriction nucleases cut DNA at specific sites that are always located between genes. B. DNA migrates toward the positive electrode during electrophoresis. C. Clones isolated from cDNA libraries contain promoter sequences. D. PCR utilizes a heat-stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured. E. Digestion of genomic DNA with AluI, a restriction enzyme that recognizes a four-nucleotide sequence, produces fragments that are all exactly 256 nucleotides in length. $F$. To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used. G. DNA fingerprinting by PCR relies on the fact that different individuals have different numbers of repeats in STR regions in their genome. H. It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same tissue.
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Which of the following statements are correct? Explain your answers. A. Restriction enzymes cut DNA at specific sites that are always located between genes. B. DNA migrates toward the positive electrode during electrophoresis. C. Clones isolated from cDNA libraries contain promoter sequences. D. PCR utilizes a heat-stable DNA polymerase because for each amplification step, double-stranded DNA must be heat-denatured. E. Digestion of genomic DNA with AluI, a restriction enzyme that recognizes a four-nucleotide sequence, produces fragments that are all exactly 256 nucleotides in length. F. To make a cDNA library, both a DNA polymerase and a reverse transcriptase must be used. G. DNA fingerprinting by PCR relies on the fact that different individuals have different numbers of repeats in STR regions in their genome. H. It is possible for a coding region of a gene to be present in a genomic library prepared from a particular tissue but to be absent from a cDNA library prepared from the same tissue.
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