Because the gal genes need to be co-regulated... O a sigma factor that recognizes gal promoters is activated by the presence of galactose O the gal genes are located in one small section of chromosome two whose expression is controlled by chromatin remodeling O the gal genes all have promoters with similar Upstream Activation Sequences O the gal genes are coordinately regulated by a single promoter that produces one long mRNA transcript that encodes multiple polypeptides
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Co-regulation means that the genes are regulated together, in a coordinated manner. Show more…
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Although operons are not common in eukaryotes, eukaryotic genes may be activated by the same stimulus. Which of the following DNA regulatory sequence makes this coordinated gene expression possible? Core promoter Enhancer element Response element Boundary element Silencer element QUESTION 18 A mutation in the gene for the yeast regulatory protein GAL4 causes yeast to grow poorly on galactose. What is the function of GAL4? It is a substrate that binds and activates a transcriptional activator. It is a product that binds and activates a transcriptional repressor. It is a transcription activator for the galactose-digesting enzyme gene. It is a transcription repressor that prevents expression of yeast galactose-digesting enzymes. It is an enzyme that metabolizes galactose.
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f) What is in common between the GAL, lac and trp gene regulatory systems? Coordinated regulation of enzymes to meet cellular metabolic needs in the context of the environment. Allosteric regulation by small molecules that are either anabolized or catabolized by the enzymes whose expression is being regulated. Inducible gene expression that conserves energy for the cell. Coupled transcription and translation. Chromatin regulation to coordinate structural gene expression. trans-acting proteins that bind cis-acting DNA sequences. Will expression of the GAL structural genes GAL1 and GAL10 be inducible, constitutive or repressed in the following genotypes of yeast: g) deltaUAS (removes UAS) h) GAL3-
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how are gal genes regulated in yeast
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