00:01
That's results from several gene transfer assay carried out to locate a regulatory sequence.
00:06
So you have three different transcription factors, x, y, and z.
00:10
They are used as probe.
00:12
Luciferase were used as a reporter protein and production of light is known as plus signal.
00:18
So no light, so i'm not, on a board, i'm not putting down anything.
00:24
So the results indicate which part of the 5dna test may have been some sort of gene regulatory role.
00:31
So how does it work? so you have, let's say, dna regions.
00:42
Some of them might be enhancer or just simply like a promoter region.
00:52
And this is the promoter.
00:56
So you can see there are five different dna, dna region.
00:59
Now a lot of time when you have a regulatory region, it is very important for the initiation of transcription.
01:10
So the goal here is to have the rna polymerase bind to the promoter region here.
01:15
But a lot of time the promoter itself is not sufficient.
01:19
Let's say one of the dna region is necessary to bring down rna polymerase, let's say five.
01:26
So in this case, if you have a certain protein bind to this region here, this is a activator protein and it is required to bring down rna polymerase.
01:49
And so when you, you can see that when you have this region that go along with the promoter, the activator is going to bring down rna polymerase.
02:01
And as a result, you'll have the reporter gene down here.
02:05
In this case, the luciferase...