Question

Below are the steps performed to make an E. coli bacteria that can produce the human insulin protein for the healthcare market. List these steps in their proper order. a. Extract the DNA from a human who does not have diabetes. b. Isolate and clone (make copies of) the gene encoding the insulin protein. c. Modify the human gene and give it a promoter that will be turned on in E. coli cells. d. Use heat shock or electroporation to introduce the recombinant human insulin gene into a living E. coli cell. e. Grow the E. coli in fermentation culture, extract the insulin protein, and sell it as Humulin.

          Below are the steps performed to make an E. coli bacteria that can produce the human insulin protein for the healthcare market. List these steps in their proper order.

a. Extract the DNA from a human who does not have diabetes.
b. Isolate and clone (make copies of) the gene encoding the insulin protein.
c. Modify the human gene and give it a promoter that will be turned on in E. coli cells.
d. Use heat shock or electroporation to introduce the recombinant human insulin gene into a living E. coli cell.
e. Grow the E. coli in fermentation culture, extract the insulin protein, and sell it as Humulin.

Added by Amparo M.

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