Question

Below is a photo of the class gel. Remember the protein standard in Lane 1 is used to establish the relationship between MW and distance. The standard is purchased from a company and the bands are proteins of known size. A standard is always run alongside the samples because without it we would not know anything about the actual size of the proteins in the other lanes. 1. Identify one house keeping protein in the tissue sample, draw a circle around it and label it. Identify one luxury protein, draw a box around it and label it. If you cannot draw on the figure due to the requirement for electronic submission you can describe the location of each band (eg. A luxury protein can be seen in LANE 24 just below the 150 kDa band) kDa 250 - 150 - 100 - 75 - 50 - 1. Standard 2. Gizzard 3. Thigh 4. Liver 5. Standard 6. Liver 7. Gizzard 8. Gizzard 9. Liver 10. Thigh 11. Thigh 12. Liver

          Below is a photo of the class gel. Remember the protein standard in Lane 1 is used to establish the relationship between MW and distance. The standard is purchased from a company and the bands are proteins of known size. A standard is always run alongside the samples because without it we would not know anything about the actual size of the proteins in the other lanes. 1. Identify one house keeping protein in the tissue sample, draw a circle around it and label it. Identify one luxury protein, draw a box around it and label it. If you cannot draw on the figure due to the requirement for electronic submission you can describe the location of each band (eg. A luxury protein can be seen in LANE 24 just below the 150 kDa band)

kDa
250 -
150 -
100 -
75 -
50 -

1. Standard
2. Gizzard
3. Thigh
4. Liver
5. Standard
6. Liver
7. Gizzard
8. Gizzard
9. Liver
10. Thigh
11. Thigh
12. Liver

Show more…

Below is a photo of the class gel. Remember the protein standard in Lane 1 is used to establish the relationship between MW and distance. The standard is purchased from a company and the bands are proteins of known size. A standard is always run alongside the samples because without it we would not know anything about the actual size of the proteins in the other lanes. 1. Identify one house keeping protein in the tissue sample, draw a circle around it and label it. Identify one luxury protein, draw a box around it and label it. If you cannot draw on the figure due to the requirement for electronic submission you can describe the location of each band (eg. A luxury protein can be seen in LANE 24 just below the 150 kDa band)

kDa
250 -
150 -
100 -
75 -
50 -

1. Standard
2. Gizzard
3. Thigh
4. Liver
5. Standard
6. Liver
7. Gizzard
8. Gizzard
9. Liver
10. Thigh
11. Thigh
12. Liver

Added by Amy W.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Madhur L

10/03/2023

Step 1

We need to identify a housekeeping protein in the tissue sample. Show more…

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Below is a photo of the class gel. Remember the protein standard in Lane 1 is used to establish the relationship between MW and distance. The standard is purchased from a company and the bands are proteins of known size. A standard is always run alongside the samples because without it we would not know anything about the actual size of the proteins in the other lanes. 1. Identify one house keeping protein in the tissue sample, draw a circle around it and label it. Identify one luxury protein, draw a box around it and label it. If you cannot draw on the figure due to the requirement for electronic submission you can describe the location of each band (eg. A luxury protein can be seen in LANE 24 just below the 150 kDa band)

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Transcript

00:01 Hello student, here the question is given and the question is about having this gel image and we have to find out this housekeeping gene and another one which is the luxury gene.

00:16 So if we want to describe this housekeeping gene as well as this luxury gene so we know this housekeeping or those genes are the one which are going to be constitutive gene.

00:30 So they are required for the basic cellular function.

00:35 So they are going to perform this basic cellular function.

00:40 So we can say they are expressed in all tissue.

00:45 So they are going to present in all tissues or the cell type because they require for this basic cellular function while in case of this luxury gene they are going to be tissue specific.

00:59 So whenever this is required they are going to only express.

01:04 So it is not going to be constantly expressed in all the tissues.

01:08 So it is going to be a tissue specific or organ specific.

01:12 So whenever it is required it is going to be expressed.

01:15 So in the particular gel we have to mark out these housekeeping gene as well as the luxury gene.

01:23 So if we look into this diagram so near this ladder so this is the molecular marker we can say the protein ladder.

01:33 So here if we look into this so this one corresponding this all having this type these 150 kd protein.

01:44 So this can be caused by a housekeeping gene because it is expressed in all type of these tissue.

01:52 So here we can say this 150 kd protein is coded by this housekeeping gene.

02:03 While if we compare others so in that case if we are looking into this 100 kd protein so here we can say in that case this one protein is going to be present in this case and below this 100 this one is going to be present...

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