Both pl and pH are important when thinking about ion exchange chromatography.
A. We are trying to purify Angiotensin I converting enzyme (ACE). This protein has a pl of 6.4. If the protein
is in a solution of pH 9.0, should we run an anion or cation exchange column? Why? (7 pts)
B. Draw the chemical functionalization that is present on this type of column. (3 pts)
C. How do we elute the protein from the column after it sticks? Be specific about what is happening
chemically. (5 pts)