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Brielle casey WARD'S SIMULATED ABO AND RH BLOOD TYPING KIT STUDENT STUDY AND ANALYSIS SHEET Shared Materials: 4 Unknown Simulated Blood Samples 1. Mr. Smith 2. Ms. Jones 3. Mr. Green 4. Ms. Brown Anti-A Simulated Typing Serum Anti-B Simulated Typing Serum Anti-Rh Simulated Typing Serum Procedure: Each team will determine the blood type of each of the four unknown blood samples. 1. Pre-label each of your four blood typing slides as follows: Slide #1: Mr. Smith Slide #2: Ms. Jones Slide #3: Mr. Green Slide #4: Ms. Brown 2. Place 3-4 drops of Mr. Smith's blood in each of the A, B and Rho wells of Slide #1. 3. Place 3-4 drops of Ms. Jones's blood in each of the A, B and Rho wells of Slide #2. 4. Place 3-4 drops of Mr. Green's blood in each of the A, B and Rho wells of Slide #3. 5. Place 3-4 drops of Ms. Brown's blood in each of the A, B and Rho wells of Slide #4. 6. Add 3-4 drops of the simulated anti-A serum in each A well on the four slides. 7. Add 3-4 drops of the simulated anti-B serum in each B well on the four slides. 8. Add 3-4 drops of the simulated anti-Rho serum in each Rho well on the four slides. 9. Use separate toothpicks to stir each sample of serum and blood. Record your observations and results in the table below. DATA TABLE 1 Agglutination Reactions Anti-A Serum | Anti-B Serum | Anti-Rh Serum | Blood Type | Observations Slide #1: Mr. Smith Slide #2: Ms. Jones Slide #3: Mr. Green Slide #4: Ms. Brown Note: A positive test is indicated by a strong agglutination reaction. See Table 2 for aid in interpreting the test results.

Brielle casey
WARD'S
SIMULATED ABO AND RH BLOOD TYPING KIT
STUDENT STUDY AND ANALYSIS SHEET
Shared Materials:
4 Unknown Simulated Blood Samples
1. Mr. Smith
2. Ms. Jones
3. Mr. Green
4. Ms. Brown
Anti-A Simulated Typing Serum
Anti-B Simulated Typing Serum
Anti-Rh Simulated Typing Serum
Procedure:
Each team will determine the blood type of each of the four unknown blood samples.
1. Pre-label each of your four blood typing slides as follows:
Slide #1: Mr. Smith
Slide #2: Ms. Jones
Slide #3: Mr. Green
Slide #4: Ms. Brown
2. Place 3-4 drops of Mr. Smith's blood in each of the A, B and Rho wells of Slide #1.
3. Place 3-4 drops of Ms. Jones's blood in each of the A, B and Rho wells of Slide #2.
4. Place 3-4 drops of Mr. Green's blood in each of the A, B and Rho wells of Slide #3.
5. Place 3-4 drops of Ms. Brown's blood in each of the A, B and Rho wells of Slide #4.
6. Add 3-4 drops of the simulated anti-A serum in each A well on the four slides.
7. Add 3-4 drops of the simulated anti-B serum in each B well on the four slides.
8. Add 3-4 drops of the simulated anti-Rho serum in each Rho well on the four slides.
9. Use separate toothpicks to stir each sample of serum and blood. Record your observations and results in the table below.
DATA TABLE 1
Agglutination Reactions
Anti-A Serum | Anti-B Serum | Anti-Rh Serum | Blood Type | Observations
Slide #1: Mr. Smith
Slide #2: Ms. Jones
Slide #3: Mr. Green
Slide #4: Ms. Brown
Note: A positive test is indicated by a strong agglutination reaction. See Table 2 for aid in interpreting the test results.

Added by Michelle H.

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