Calculate the DNA concentration in µg/mL from the following information: a. Absorbance reading at 260 nm from a 1:100 dilution = 0.307
Added by Alberto L.
Step 1
The concentration of DNA can be calculated using the Beer-Lambert law, which relates the absorbance of a substance to its concentration. For DNA, an absorbance of 1.0 at 260 nm corresponds to a concentration of 50 µg/mL in a 1 cm pathlength cuvette. Show more…
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Calculate the nucleic acid concentration in µg/mL from the following information: (4pts) DNA: A reading at 260 nm = 0.307 RNA: A reading at 260 nm = 0.307 DNA: A reading at 260 nm from a 1:100 dilution = 0.086 DNA: A reading at 260 nm from a 1:50 dilution = 0.172
Jenny W.
What is the concentration of DNA in a solution if its absorbance at 260 nm is 0.64? What is the expected absorbance of a DNA solution with a concentration of 150 ng of DNA/μL of solution?
Jhankar T.
Remember, an absorbance or optical density (OD) OD260 of 1 indicates a concentration of 50µg/mL DNA. Therefore, DNA concentration = OD260 x 50 x Dilution Factor dsDNA concentration = 50 µg/mL × OD260 × dilution factor
Sukhada S.
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