Calculate the total quantity of drug that had dissolved (mg). Note that although the volume in the vessel did not change because retrieved media was replaced for each sample, the drug contained in each 3 mL retrieved sample was not returned to the vessel. The calculation must take this into account. (= quantity at step 3 + sum of step 4 from the first sample to the previous sample). Use the following data:
The absorbance was 0.317 at 1 min, Step 1: the concentration of drug in the diluted sample (mg/100ml) was 0.456180745, Step 2: the quantity of drug in the 50mL volumetric flask (mg/50mL) was 0.22809373,Step 3: the quantity of drug dissolved in the 900mL vessel (mg/900mL) was 8.211253410 and Step 4: used the answer to step 2 to calculate the quantity of drug that was dissolved in the 3 mL retrieved sample (mg/3 mL) which was 0.027370845