00:01
So transformation is a process in which it involves the change in genetic material of an organism due to the incorporation of dna from the environment that is the egg environment that is the egg.
00:44
Exogenous dna.
00:48
So if a foreign dna is picked up from the environment, that is the exogenous dna by a given organism which changes the genetic component of this organism, then this process is known as transformation.
01:07
So transformation is seen in bacteria very actively where the bacterial cells can pick up exogenous or foreign dna from outside, that is from the environment into its own cytoplasm.
01:25
So now, since the bacteria can pick up the foreign dna, therefore we can use the principle of transformation.
01:37
So this e.
01:39
Coli, so the bacteria e.
01:43
Coli can undergoes can undergo something that we do is.
01:46
Is that is the gfp transformation.
01:51
So in this experiment, what is done, so gfp stands for green fluorescent protein.
02:09
So this green fluorescent protein is derived from a particular jellyfish and this fluorescence which is provided by this protein glows the equal.
02:23
In the uv light.
02:26
So since this gfp and so in a given plasmid this gfp and an ampicillin resistant gene is added to the plasmid of an ecoli so both these genes are added to the plasmid of ecoli now this plasmid of ecoli now this plasmid of a coli.
02:52
Now this plasmid of a e.
02:56
Coli and some e.
02:58
Coli cells are allowed to transform.
03:01
So the e.
03:02
Coli undergoes transformation.
03:10
Now the cells or the e.
03:12
Coli cells which gets transformed.
03:15
So we can consider that there can be two case scenarios in this case.
03:21
So in the first case scenario, the e...