Use the data for the following four hypothetical microscopes to answer questions 12-14 Microscope Number Objective Lens Ocular Lens 1 2 3 4 25X 20X 20X 40X 5X 15X 10X 5X If a slide showing the same types of living organisms is examined with each of the microscopes, in which two microscopes will the microbes appear to move with the same degree of speed? A. Microscopes 1 and 2 D. Microscopes 2 and 4 B. Microscopes 2 and 3 E. Microscopes 3 and 1 C. Microscopes 3 and 4 Given that each slide had the same density of microbes, with which microscope would you expect to observe the greatest number of microbes at any given instant? A. Microscope 1 C. Microscope 3 B. Microscope 2 D. Microscope 4 14. If you were observing a cell from you cheek under each of the microscopes, in which microscope would this cheek appear to be the largest? A. Microscope 1 C. Microscope B. Microscope 2 D. Microscope 4
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Step 1: Calculate the total magnification for each microscope by multiplying the objective lens magnification by the ocular lens magnification. Show more…
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When using the light microscope: A- You always start with a high power and work your way down in magnification because the microscopes we have are parfocal. B- You always use immersion oil with the 40X objective to get the best image. C- Organisms are usually stained when using brightfield microscopy. D- The advantage of darkfield is being able to view live, unstained (and sometimes motile) cells.
Adi S.
Which of the following changes would increase resolution when using the oil immersion lens? Choose all that are correct. a. Adjust fine focus knob. b. Use immersion oil. c. Increase rheostat intensity. d. Raise condenser. e. Lower condenser. f. Open iris diaphragm. g. Close iris diaphragm. Answer choices: A. a, c, e, f B. a, b, e, g C. a, b, c, d, f D. b, c, d, e 2. Why must immersion oil be used with the oil immersion lens? A. To increase contrast. B. To increase light refraction to decrease contrast. C. To decrease light refraction thus increasing resolution. D. To increase light refraction to increase resolution. 3. To focus a specimen, it is best to start with the ___________ objective lens. A. Oil immersion. B. Ocular. C. High dry. D. Scanning. 4. What is the function of the iris diaphragm and substage condenser? A. To focus using oil immersion lens. B. To move slide left to right. C. To move stage up and down. D. When opened and raised, increases light focused on specimen thus increasing resolution. 5. When using the high dry or oil immersion lens, which focus adjustment knobs should never be used? A. Fine focus adjustment knob. B. Medium focus adjustment knob. C. Low focus adjustment knob. D. Coarse focus adjustment knob.
1. What is the total magnification of a compound light microscope with an objective lens magnification of 40X and an ocular lens of 10X? 2. Why is immersion oil necessary at 1000X but not with the lower power objectives? 3. What are the advantages of brightfield, darkfield, and phase-contrast microscopy? 4. Why won't other bacteria fluoresce in the FTTA-ABS test? 5. What is the principal use of SAM? 6. How do TEM and SEM images of the same organism differ? 7. What is the principle employed in scanned-probe microscopy? 8. How can the Gram reaction be useful in prescribing antibiotic treatment? 9. Why is Mycobacterium tuberculosis easily identified by the acid-fast stain? 10. Of what value are capsules, endospores, and flagella to bacteria?
Dominador T.
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