Can the amino acid sequence of a polypeptide chain be used to determine the nucleotide sequence of the mRNA that encodes it? Explain
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If you were given an amino acid sequence, would you be able to deduce the exact nucleotide sequence of the mRNA transcript that was used to produce it? Explain the basis of your answer.
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Determining the amino acid sequence in a protein usually involves treating the protein with various reagents that break up the protein into smaller fragments, which can be individually sequenced. Treating a particular 11 -amino-acid polypeptide with one reagent produced these fragments: trp-glu-val, gly-arg, ala-ser-phe-gly-asn-lys Treating the same polypeptide with a different reagent produced the following fragments: gly-asn-lys-trp, glu-val, gly-arg-ala-ser-phe What is the amino acid sequence of the polypeptide?
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