Can you please help me with this question? NOTE that the last student number digit is: 8
Thank you in advance
Question 1 (8 points)
You have recently discovered the signaling pathway underlying locomotory activity Term Test #3: Q1
ABC pathway parameters
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patterns in the nematode worm C elegans. These patterns involve alternating times
of high activity levels and low activity levels. You have identified the signaling
pathway that controls these oscillating activity levels; it can be reduced to a model
network described by three interacting components (A, B, and C). You know nothing
about A, B, and C, other than their interaction are required for the observed activity
patterns. So, you set out to determine how each component interacts with the
others (i.e. how do A, B, and C influence one another to generate these oscillations).
You are able to reduce the number of hypotheses to the following list:
A inhibits B, B inhibits C, and C inhibits A
A inhibits B, B inhibits C, and C activates A
A activates B, B activates C, and C inhibits A
A inhibits B, B activates C, and C activates A
A activates B, B inhibits C, and C activates A
This simulator allows you to test your hypotheses. Note that the simulator gives you
access to a sensitivity parameter, the degradation rates for A, B, and C, and the
ability to hold A constant at different concentrations (i.e. the CLAMP feature, that
you can turn ON and OFF by setting the parameter ON=1 or OFF=0).
Be sure to SELECT the LAST DIGIT of your student number in the appropriate box to
initialize the simulator for all your experiments, before pressing RUN.
Use an experimental approach to determine which of the original five hypotheses
listed above is most likely to be true. Copy-paste the appropriate hypothesis from
the above list to indicate your choice.
Then briefly describe the strategy you used to determine your choice.
inM
Term Test #3: Q1 ABC pathway parameters
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sensitivity (of all interactions 1 A degradation rate 1 B degradation rate 1 C degradation rate 100 Total time (hr)
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Select last digit of your student number
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Experimentally clamp concentration of A (if ON, clamp begins at 20 hours) 0 clamp OFF=0, ON=1 0 clamp concentration (nM
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+ C
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Run
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