Case #1: A mother took her child to his doctor with a wound on his elbow. The doctor suspects that the child is suffering from a staphylococcal infection. In an attempt to isolate the pathogen, the doctor takes a sample of the wound and attempts to culture it on general purpose media incubated at 25oC. After incubation, the plate showed a number of different types of bacteria besides the potential staphylococcal species. Discussion Questions 1. What would you recommend the doctor should do to reduce the number of different types of bacteria on the general purpose media plate? 2. What would you recommend the doctor should do to only isolate the staphylococcal species from the child's wound without any of the other bacterial types?
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1. The plate pictured is from a past student. What is the most likely conclusion? Select one: a. The results are a classic example of Staphylococcus resistance. b. Bacitracin is bactericidal and based on streak pattern, there must be contamination. c. The colonies are a different color at the edge of the zone than the rest of the lawn. d. Could be susceptible, but possible extended incubation time allowed bacteriostatic effects to decrease. e. The bacitracin disk used had no potency. 2. These pictures are from past students who used the same sample. What is the most likely interpretation of the unknown bacterium results? Select one: a. Susceptible b. Resistant c. Large clearing d. Undetermined e. Streptococcus 3. The plate pictured is from a past student. What is the primary mistake? Select one: a. The bacterial lawn was not large enough. b. Bacitracin is not effective on this plate. c. No bacteria were added to the plate. d. The bacitracin disk is blue. e. The plate was probably not incubated.
Shaiju T.
Case Study 1: Parents bring their 2-year-old infant into the emergency room of a clinic. The infant is suffering from diarrhea and vomiting, and initial observation shows a dangerous level of dehydration. Initial tests do not indicate a bacterial infection or the presence of bacterial toxins. Discussion: Ruling out the involvement of a bacterial pathogen, discuss what other pathogens may be involved (see Reoviridae and Caliciviridae). Discuss the characteristics of these viruses and their associated diseases.
Ripujeet P.
Case Study 1: Parents bring their 2-year-old infant into the emergency room of a clinic. The infant is suffering with diarrhea and vomiting, and initial observation shows a dangerous level of dehydration. Initial tests do not indicate a bacterial infection or the presence of bacterial toxins. Discussion Ruling out the involvement of a bacterial pathogen, discuss what other pathogens may be involved (see Reoviridae and Caliciviridae). Discuss the characteristics of these viruses and their associated diseases.
Sulav P.
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