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CASE HISTORY #5 Directions: Read the following case history and answer the questions, using your text, any available reference books and information from your lab exercises, as well as your notes from both the lab and lecture material. (Note: questions are worth 1 point each) A 30 year old sexually active woman was seen in the emergency room of a local hospital. She complained that she had burning while urinating and that it had been occurring for more than one week. A clean catch urinalysis with culture was ordered by the physician. The UA results were as follows: Glucose - negative Bilirubin - negative Ketones - negative Specific gravity - 1.015 Blood - 1+ pH - 7.5 Protein - 1+ Urobilinogen - normal Nitrite - positive Leukocytes - 2+ (moderate) Microscopically many red blood cells and white blood cells and many rod shaped bacteria were seen. The urine was sent to the microbiology lab and it was plated on EMB / BAP biplates with calibrated loops; 1 µl and 10 µl and incubated for 24 hours at 37°C. Greater than 10,000 colonies (> 10^5 CFU per mL) were seen on both biplates after 24 hours of incubation. The colonies had a green sheen on the EMB side and showed large grey colonies with slight hemolysis on the BAP side. The Gram stain result revealed Gram negative rods occurring singly and in pairs. The oxidase test was negative. Antibiotic Sensitivity tests were performed with the standard antibiotics used for urinary tract infections caused by Gram negative bacteria. 1. List some of the bacteria that could be responsible for this UTI (urinary tract infection). 2. Which one of the bacteria is the most common cause of UTI's? 3. How is this type of infection transmitted? 4. What antibiotics would be the most effective for treating this infection? 5. List three references used to answer these questions. At least one reference should be a textbook. These will vary, here are a few I use:

CASE HISTORY #5

Directions: Read the following case history and answer the questions, using your text, any available reference books and information from your lab exercises, as well as your notes from both the lab and lecture material. (Note: questions are worth 1 point each)

A 30 year old sexually active woman was seen in the emergency room of a local hospital. She complained that she had burning while urinating and that it had been occurring for more than one week. A clean catch urinalysis with culture was ordered by the physician.

The UA results were as follows:
Glucose - negative
Bilirubin - negative
Ketones - negative
Specific gravity - 1.015
Blood - 1+
pH - 7.5
Protein - 1+
Urobilinogen - normal
Nitrite - positive
Leukocytes - 2+ (moderate)

Microscopically many red blood cells and white blood cells and many rod shaped bacteria were seen.
The urine was sent to the microbiology lab and it was plated on EMB / BAP biplates with calibrated loops; 1 µl and 10 µl and incubated for 24 hours at 37°C.

Greater than 10,000 colonies (> 10^5 CFU per mL) were seen on both biplates after 24 hours of incubation. The colonies had a green sheen on the EMB side and showed large grey colonies with slight hemolysis on the BAP side. The Gram stain result revealed Gram negative rods occurring singly and in pairs. The oxidase test was negative.

Antibiotic Sensitivity tests were performed with the standard antibiotics used for urinary tract infections caused by Gram negative bacteria.

1. List some of the bacteria that could be responsible for this UTI (urinary tract infection).

2. Which one of the bacteria is the most common cause of UTI's?

3. How is this type of infection transmitted?

4. What antibiotics would be the most effective for treating this infection?

5. List three references used to answer these questions. At least one reference should be a textbook.
These will vary, here are a few I use:

CASE HISTORY #5

Directions: Read the following case history and answer the questions, using your text, any available reference books and information from your lab exercises, as well as your notes from both the lab and lecture material. (Note: questions are worth 1 point each)

A 30 year old sexually active woman was seen in the emergency room of a local hospital. She complained that she had burning while urinating and that it had been occurring for more than one week. A clean catch urinalysis with culture was ordered by the physician.

The UA results were as follows:
Glucose - negative
Bilirubin - negative
Ketones - negative
Specific gravity - 1.015
Blood - 1+
pH - 7.5
Protein - 1+
Urobilinogen - normal
Nitrite - positive
Leukocytes - 2+ (moderate)

Microscopically many red blood cells and white blood cells and many rod shaped bacteria were seen.
The urine was sent to the microbiology lab and it was plated on EMB / BAP biplates with calibrated loops; 1 µl and 10 µl and incubated for 24 hours at 37°C.

Greater than 10,000 colonies (> 10^5 CFU per mL) were seen on both biplates after 24 hours of incubation. The colonies had a green sheen on the EMB side and showed large grey colonies with slight hemolysis on the BAP side. The Gram stain result revealed Gram negative rods occurring singly and in pairs. The oxidase test was negative.

Antibiotic Sensitivity tests were performed with the standard antibiotics used for urinary tract infections caused by Gram negative bacteria.

1. List some of the bacteria that could be responsible for this UTI (urinary tract infection).

2. Which one of the bacteria is the most common cause of UTI's?

3. How is this type of infection transmitted?

4. What antibiotics would be the most effective for treating this infection?

5. List three references used to answer these questions. At least one reference should be a textbook.
These will vary, here are a few I use:

Added by Sarah K.

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