1- Which of the following is TRUE about the protein, HIF1-α
(choose ALL that apply)?
a- It does not undergo hydroxylation when oxygen levels are
low.
b- It is ubiquitinated when oxygen levels are normal.
c- It functions as a transcription factor when oxygen levels are
low.
d- It is bound by the protein, Keap1 when oxygen levels are
normal.
Which of the following is true about the nucleotide binding
sites within AMPK (choose ALL that apply)?:
a- Site 3 can bind either ATP or ADP.
b- Site 4 is occupied by AMP only under severe stress
conditions.
c- AMPK's kinase activity is highest when Site 1 is occupied by
AMP.
d- AMPK's kinase domain is located within Site 2.
3- The protein, ____________ acts as a 'sensor' for ________ due to
the presence of several __________ residues that become ___________
during ___________.
a- HIF alpha; proline amino acids; hydroxylated; hypoxia
b- AMPK; low ATP levels; cysteine amino acids; phosphorylated;
oxidative stress
c- Keap1; ROS; cysteine amino acids; oxidized; oxidative
stress
d- None of the above.
4- When ATP levels are low, the enzyme, ___________
converts _________ to ____________.
a- AMPK; ATP; AMP + 2ADP
b- Adenylate kinase; 2ADP; 2ATP
c- TOR; AMP; ADP
d- Adenylate kinase; 2ADP; ATP + AMP