Compare the distribution of cells among G1, S, and G2 phases in the control and treated samples. Which statement best describes the difference(s) in the distribution of cells in the treated sample compared to the control sample?
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The cell cycle consists of three main phases: G1 (Gap 1), S (Synthesis), and G2 (Gap 2). G1 is the phase where the cell grows and prepares for DNA replication, S is where DNA replication occurs, and G2 is where the cell prepares for mitosis. Show more…
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Supreeta N.
If cells are grown in a culture medium containing radioactive thymidine, the thymidine will be covalently incorporated into the cell's DNA during S phase. The radioactive DNA can be detected in the nuclei of individual cells by autoradiography (i.e., by placing a photographic emulsion over the cells, radioactive cells will activate the emulsion and be labeled by black dots when looked at under a microscope). Consider a simple experiment in which cells are radioactively labeled by this method for only a short period (about 30 minutes). The radioactive thymidine medium is then replaced with one containing unlabeled thymidine, and the cells are grown for some additional time. At different time points after replacement of the medium, cells are examined in a microscope. The fraction of cells in mitosis (which can be easily recognized because the cells have rounded up and their chromosomes are condensed) that have radioactive DNA in their nuclei is then determined and plotted as a function of time after the labeling with radioactive thymidine (Figure Q18-14). A. Would all cells (including cells at all phases of the cell cycle) be expected to contain radioactive DNA after the labeling procedure? B. Initially there are no mitotic cells that contain radioactive DNA (see Figure Q18-14). Why is this? C. Explain the rise and fall and then rise again of the curve. D. Estimate the length of the G2 phase from this graph.
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You are investigating cell division among three cell samples. The graph below is from a study that includes the length of time spent in each phase of the cell cycle by the cells. Use the graph to answer the following questions 1-3. (Note: the time that delta samples spend in M phase is 0). Analysis of time spent in each cell cycle phase Based on the graph, which of the following statements below is the best conclusion concerning the difference between the S phases for beta and gamma? Please circle your answer and provide your reasoning for the answer choice. (No reasoning will result in only partial credit). (1 pt.) A) gamma contains more DNA than beta B) beta and gamma contain the same amount of DNA C) beta cells reproduce asexually D) beta is a plant cell and gamma is an animal cell
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