Question

Complete the data missing from the table, which shows assay data from the purification protocol of a hypothetical protein. Assay data Crude extract (NH4)2SO4 precipitation DEAE-cellulose chromatography Gel-filtration chromatography Affinity chromatography Total protein (mg) 20,000 5000 1500 500 45 Total activity (units) 4,000,000 3,000,000 1,000,000 750,000 675,000 Specific activity (units mg-1) Purification level 1 Yield (%) 100

          Complete the data missing from the table, which shows assay data from the purification protocol of a hypothetical protein.

Assay data	Crude extract	(NH4)2SO4 precipitation	DEAE-cellulose chromatography	Gel-filtration chromatography	Affinity chromatography
Total protein (mg)	20,000	5000	1500	500	45
Total activity (units)	4,000,000	3,000,000	1,000,000	750,000	675,000
Specific activity (units mg-1)					
Purification level	1				
Yield (%)	100

Complete the data missing from the table, which shows assay data from the purification protocol of a hypothetical protein.

Assay data Crude extract (NH4)2SO4 precipitation DEAE-cellulose chromatography Gel-filtration chromatography Affinity chromatography
Total protein (mg) 20,000 5000 1500 500 45
Total activity (units) 4,000,000 3,000,000 1,000,000 750,000 675,000
Specific activity (units mg-1)
Purification level 1
Yield (%) 100

Added by Pamela J.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Madhur L

09/17/2022

Step 1

The total protein in the crude extract is 20,000 mg and the total activity is 4,000,000 units. Therefore, the specific activity (units/mg) is 4,000,000 units / 20,000 mg = 200 units/mg. Show more…

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Complete the data missing from the table, which shows assay data from the purification protocol of a hypothetical protein. Assay data Crude extract (NH4)2SO4 precipitation DEAE-cellulose chromatography Gel-filtration chromatography Affinity chromatography Total protein (mg) 20,000 5000 1500 500 45 Total activity (units) 4,000,000 3,000,000 1,000,000 750,000 675,000 Specific activity (units mg-1) Purification level 1 Yield (%) 100