Question

Consider the following restriction enzyme sites EcoRI 5'GAATTC 3'CTTAAG Kpn1 5'GGTACC 3'CCATGG BamHI 5'GGATCC 3'CCTAGG SalI 5'GTCGAC 3'CAGCTG HindIII 5'AAGCTT 3'TTCGAA Which of the restriction enzymes generates 3' overhangs Select one: a. EcoRI, BamHI, Sall, HindIII b. All of them because for every 5' overhang there must be a corresponding 3' overhang c. None of them d. Kpnl 

          Consider the following restriction enzyme sites
EcoRI
5'GAATTC
3'CTTAAG
Kpn1
5'GGTACC
3'CCATGG
BamHI
5'GGATCC
3'CCTAGG
SalI
5'GTCGAC
3'CAGCTG
HindIII
5'AAGCTT
3'TTCGAA
Which of the restriction enzymes generates 3' overhangs
Select one:
a. EcoRI, BamHI, Sall, HindIII
b. All of them because for every 5' overhang there must be a corresponding 3' overhang
c. None of them
d. Kpnl
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Consider the following restriction enzyme sites EcoRI 5'GAATTC 3'CTTAAG Kpn1 5'GGTACC 3'CCATGG BamHI 5'GGATCC 3'CCTAGG SalI 5'GTCGAC 3'CAGCTG HindIII 5'AAGCTT 3'TTCGAA Which of the restriction enzymes generates 3' overhangs Select one: a. EcoRI, BamHI, Sall, HindIII b. All of them because for every 5' overhang there must be a corresponding 3' overhang c. None of them d. Kpnl

Added by Victor T.

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Biology for AP Courses
Biology for AP Courses
Julianne Zedalis, John Eggebrecht
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Consider the following restriction enzyme sites: EcoRI KpnI BamHI SalI HindIII 5'GAATTC 3'CTTAAG 5'GGTACC 3'CCATGG 5'GGATCC 3'CCTAGG 5'GTCGAC 3'CAGCTG 5'AAGCTT 3'TTCGAA Which of the restriction enzymes generates 3' overhangs? Select one: a. EcoRI; BamHI; SalI, HindIII b. All of them because for every 5' overhang there must be a corresponding 3' overhang c. None of them d. KpnI
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Transcript

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00:01 Hello students, the question that is given over here is says that we have to consider the following restriction enzymes given over here.
00:18 And we have to determine which of the restriction enzyme given below generates 3 prime overhand.
00:29 The right answer for this question is option a, e -cori, b -a -m -h -i, s -a -i -i -i -i -h -i and h -i -n -d.
00:59 These are the restriction enzymes that generate the 3 prime overhand.
01:11 Over here, restriction enzymes that leave the dna asymmetrically leave several single -standard bases.
01:37 If the single -standard basis end with 3 -prime phosphate group, then the enzyme is said to have 3 -prime overhead...
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