Consider the plasmid extraction process/protocol. What is the main purpose of the buffer that makes the solution very alkaline (buffer P2)? Choice 1 of 4:precipitate the genomic DNA and cellular membranes Choice 2 of 4:destroy membranes and proteins, break H-bonds Choice 3 of 4:separate plasmid DNA from genomic DNA Choice 4 of 4:wash away proteins and small RNA or DNA fragments
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Adi S.
Match the buffer used in plasmid isolation with the correct mechanism (mode of action). Alkaline Lysis Washes DNA Destabilizes outer membrane of bacterial cells Elutes DNA Tightens DNA bound to silica in the column Denature Proteins and precipitate formation Buffer EB Buffer N3 Buffer P1 Buffer P2 Buffer PB Buffer PE
Madhur L.
What is the primary purpose of the lysis buffer in DNA extraction procedures? to remove the remnants of any cellular components that might interfere with the polymerase chain reaction for barcoding binding to DNA to isolate it from the other cell components physical breakdown of hard structures of cells, like cell walls chemical breakdown of the membranes within the cells
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