5. Consider the polypeptide whose primary structure is shown below. Gly-Ile-Lys-Cys-Glu-Tyr-Val-Arg-Gly-Leu | S | S | Lys-Tyr-Cys-Glu-Val a) What peptide fragments are produced by trypsin digestion? b) What peptide fragments are produced by trypsin after reduction of the disulfide bond? c) What peptide fragments are produced by digestion with chymotrypsin? d) What amino acid(s) would be the first derivatized when the polypeptide is reacted with phenylisothiocyanate?
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Trypsin cleaves at the C-terminal side of lysine (K) and arginine (R) residues. ** Show more…
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