00:01
When we speak about pcr, what we first need to understand that is that it is inclusion of polymerase chain reaction.
00:10
Now, what is polymerase chain reaction? as the name suggests, it is a chain reaction including the polymerase, which means a single copy of dna can be amplified into multiple coffees with the action of pcr.
00:29
Now, pcr has three steps, denaturation written as de, annealing written as ann and extension written as ex.
00:38
So, this comprises of one cycle.
00:43
And there could be several cycles of pcr in a given period of time in order to amplify the dna to many copies in particular.
00:55
Now coming to the given paragraph.
00:57
So first it says that pcr particularly is a powerful tool used to isolate and make large quantities of defined dna fragment.
01:09
This is true.
01:11
So there is no change in this.
01:13
So it makes dna fragment from a complex genome.
01:18
So pcr is a powerful tool used to amplify and make large quantities of dna fragment from a complex genome is correct.
01:25
So coming to the second statement.
01:27
Second statement says that pcr particularly takes advantage of electrophoresis written as e and synthetic oligo nucleotides written as ol particularly.
01:41
So that is also true because there is synthetic oligonucleotides and it also goes through the process of electrophoresis for the occurrence of several copies of dna.
01:52
Next coming to the statement which states that it starting it's starting, starting material can be as small as a single cell...