Protein targeting Signal sequence recognized by SRP Free ribosomes Targeted after translation (TAT) Ribosome goes to rough ER (SRP binds to SRP receptor passed to translocati Translation, folding, modification in ER Destination: Nucleus Golgi Destination: TAT: No signal sequence TAT: Internal signal TAT: N- terminal signal Destination: Mitochondria Cytosol and Destination: Destination: chloroplasts Lysosome Dest: Dest: Secreted from cell Endosome PM
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Protein targeting Free ribosomes Golgi apparatus Protein is imported into mitochondria Lysosome Internal nuclear localization signal N-terminal mitochondrial signal ER Signal sequence recognized by SRP Protein is imported into nucleus Ribosome moves to rough ER Protein stays in the cytosol Plasma membrane Secreted from cell (default) No signal sequence Translation resumes and protein imported into ER Endosome
Madhur L.
Make a flowchart that traces the movement of a secreted protein from its site of synthesis to the outside of a eukaryotic cell. Identify all the organelles that the protein passes through. Add notes indicating what happens to the protein at each step.
Order the steps below for a secreted protein. This is the order of the path for a secreted protein from gene expression to secretion outside the cell . Use 1 for step 1 and so on. protein is threaded through ER transmembrane channel Transcription factors bind the gene promoter and RNA polymerase carries out transcription SRP positions ribosome on transmembrane channel Translation stops when the signal sequence on protein is bound by SRP vesicle with protein inside blebs off from golgi and fuses with cell membrane SRP leaves and protein synthesis continues vesicle with protein inside blebs off from ER and fuses with golgi mRNA is processed and transported out of the nucleus and translated on free ribosome protein ends up in lumen of ER protein is released outside the cell SRP binds to SRP receptor on ER membrane surface
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