CSUSM BIOL 210 Lab Introduction to Cellular and Molecular Biology Obtain the class data and complete the table. For the class data, you only need to record the % change in mass and not the initial/final masses. Table: Observations and results of osmosis experiment with glucose Trial Initial mass (g) Final mass (g) % change Average concentration % change in mass 1 35.49 34.719 -1.0 24.9 25.632 2 0.2M 239 232.49 21.254 5.52 3 0.44M 262 30 12.12 26.2 4 0.6M 28.6 85.02 20.3 32.435 5 0.42M 204.2 9 20.9 Plot the sucrose concentration on the X-axis and the mass change on the Y-axis. Use Microsoft Excel to generate a scatter plot. You will need to obtain the class averages for each sucrose concentration and percent change. Be sure to properly label the axes for each sucrose concentration and percent change in mass. Results: Include a trendline regression (including units) on the graph for this week's equation and R² value.
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First, we need to organize the given data into a table format. The table should have columns for sucrose concentration, initial mass, final mass, and % change in mass. Show more…
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Table 1. Data for Experiment Estimating Osmolarity of Potato cells by Change in Weight Sucrose Molarity 0.0 M | 0.1M | 0.2M | 0.3M | 0.4M | 0.5M | 0.6M Final weight (g): 1.42 | 1.75 | 2.13 | 2.09 | 1.34 | 1.42 | 1.85 Initial weight (g): 1.20 | 1.55 | 2.00 | 2.02 | 1.45 | 1.65 | 2.33 Weight change: 0.22 | 0.20 | 0.13 | 0.07 | -0.11 | -0.23 | -0.48 % change in weight: 18.33 | 12.90 | 6.50 | 3.46 | -7.59 | -13.93 | -20.60 6. (1.5 pts) Above is a table of raw data from the experiment to estimate the osmolarity of potato cells. Based on these data, what is the best estimate of the molarity of potato cells? The potato with a high solute concentration gained weight compared with the potato with a low solute concentration. 7. (1.5 pts) How did you derive your conclusion for your answer to question 6? The concentration of water moves from a high concentration to a low concentration areas to equalize the water potential.
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Table 3. Sucrose concentration in bags A, B and C vs. Rate of mass change over 60 minutes. Sucrose concentration | Rate of mass change over time (%/min) 15% (A) | -0.833 30% (B) | -0.229885 65% (C) | 0.52341 f. Graph the rate of change in mass versus sucrose concentration. g. Bags A and B both lost mass over the 60 minutes, but the rate of mass change in A was faster than in B. How is this evident from the graph above and what does it suggest about these solutions? h. Determine the isotonic concentration of sucrose. Remember, the isotonic concentration is that at which no net osmosis is happening and therefore the rate of change in mass or rate of osmosis is equal to zero. i. What is the concentration of the solution in the beakers? Explain.
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3. The following data were measured for a green coloured solution of Pope-Y. Trial Concentration (M) Absorbances 1 0.20 0.27 2 0.30 0.41 3 0.40 0.55 4 0.50 0.69 Unknown 0.32 a) Plot a graph, and include it below, then determine the unknown concentration based on the data given. Show your calculations. b) The student handles the cuvette and leaves fingerprints in the path of the light beam. How will this affect the calculated concentration of the unknown? Explain your answer. c) Why is this method of determining the concentration of Pope-Y solution appropriate, whereas using the same method for measuring the concentration of NaCl solution would not be appropriate?
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