d) A plasmid used as a cloning vector has the reporter genes tetR (tetracycline resistance) and lacZ (?-galactosidase) with restriction sites for cloning DNA fragments within lacZ. What should be added to the growth medium to select for bacterial cells with recombinant vectors, and what color will the colonies of recombinant cells be? a. Tetracycline and X-Gal; blue b. Tetracycline and ?-galactosidase; white c. Tetracycline and X-Gal; white d. X-Gal only; blue e. Tetracycline only; white e) Suppose that you want to express a protein from animal cells using recombinant DNA technology. Would you use a bacterium, virus, or yeast? Explain your answer. f) Cloning of a human growth hormone required construction of a cDNA in order to be clone it into a bacterial host cell. What is a cDNA and why was this strategy possibly used? Explain.
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d) To select for bacterial cells with recombinant vectors, we need to add tetracycline to the growth medium, as it will allow only the cells with the tetracycline resistance gene (tetR) to grow. Show more…
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A recombinant DNA was constructed by inserting the DNA of interest into the AmpR gene of a pBR322 vector. The vector also contains a TetR gene that is not interrupted. After introducing the recombinant DNA into bacterial cells, the resulting transformants were tested on a variety of media. Which of the following results would be expected? A)no growth on either ampicillin or tetracycline containing media B) growth on ampicillin but not tetracycline containing media C) growth on tetracycline but not ampicillin containing media D) growth only on media containing both antibiotics E) growth on both ampicillin containing media and tetracycline containing media
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A plasmid suitable for use as a cloning vector must contain which two features? a. a gene for positive selection, such as an antibiotic resistance gene, and a sequence that recognizes an activator b. a restriction enzyme cleavage site and a gene for positive selection, such as an antibiotic resistance gene c. a gene for a restriction endonuclease and a gene for positive selection, such as an antibiotic resistance gene. d. an operator and a promoter e. a restriction enzyme cleavage site and an operator and a gene for a repressor protein
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