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Hello students, some bacteria produce endospores to survive in unfavorable conditions such as drought, low nutrient condition, radiation, high temperature, osmotic pressure, various chemical disinfectants.
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They do so by producing dormant and highly resistant cells to preserve the genetic material of cell.
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Answer to the next question, heating is very important as it helps to overcome the high keratin content protein coat which surrounds the spore.
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This will make stain able to go and bind the endospore.
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Answer to the next question, the two types of bacteria that produce endospores are the first is bacillus cereus.
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This is a kind of bacteria which is anaerobic, spore forming and commonly found in soil, on vegetable and many raw and processed food.
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This bacteria is important as it cause food poisoning.
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Next type is clostridium botulinum.
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This clostridium botulinum is an anaerobic spore forming gram positive bacillus which is ubiquitous in environment.
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This botulism is a rare but serious illness caused by a toxin that attack the body's nerves and cause difficulty breathing, muscle paralysis and even death because when this bacteria grows in food it produce toxin in food.
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Answer to the next question, if the paper solution had not been heated then it is likely that the endospore would not have been stained because without heating the stain may have not able to bind to the endospore.
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Answer to the next question, the endospore's tad grave on the petri dishes would most likely to be aerobic because clostridium is anaerobic and bacillus is facultative anaerobic which means that when they are growing in oxygen which is not a favorable atmosphere or environment for them so in this case they will produce endospores that's why they are aerobic...