'Describe a toeprint assay involving mammalian ribosomal subunits and an mRNAin a cell-free extract that contains all the factors necessary for translation (a) What results would you expect to sce with the 40Sribosomal subunits alone? (b) With 60S subunits alone? (c) With both subunits and all amino acids except serine, which is required in the 15th position of the polypeptide?'
Added by Iker A.
Step 1
A toeprint assay is a technique used to determine the position of ribosomes on an mRNA molecule during translation. It involves using a primer that anneals to the mRNA upstream of the ribosome binding site (RBS) and extends towards the start codon. The ribosome Show more…
Show all steps
Your feedback will help us improve your experience
Adi S and 78 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Describe the process of protein synthesis from mRNA. What amino acid sequence would you expect from each of the following mRNA segments? a. - AUA CCC UUG GAU- b. - CCU CGC ACG CCA UGA- c. - AUG CAC AAG GAA GUG CUG -
Nucleic Acids and Protein Synthesis
Protein Synthesis
22. In eukaryotes binding of the 40S ribosomal subunit to mRNA: a) follows the binding of the 60S subunit b) involves base-pairing between the AUG start codon and rRNA c) involves ribosomal proteins binding to a recognition sequence in the mRNA d) involves base-pairing between the mRNA and rRNA e) involves recognition of the 5' terminus of the mRNA by the 40S subunit
Md.Daniyal A.
It is possible to synthesize proteins in vitro (in a test tube) without the use of living cells. If starting from a mature mRNA transcript, which of the following components would not be needed to do this? A. Adenine molecules. B. Amino acids. C. Ribosomes.
Madhur L.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD