00:01
Hello everyone, let's get started.
00:03
So the question is about pcr.
00:06
So pcr means a polymerase chain reaction.
00:15
So it is a technique used to amplify small segments of dna.
00:30
So sometimes we call it as a molecular photocopying.
00:41
The polymerous chain reaction is a fast and inexpensive technique and is used to add.
00:48
Amplifier copy small segments of dna because significance amount of a sample of dna are necessary for molecular as well as genetic analysis.
01:04
So that's why we use this technique.
01:08
It is one of the most important scientific advances in molecular biology and bcr revolutionized the study of dna, such an extended creator, carrie p.
01:23
Mullis was awarded the nobel prize for chemistry in 1993.
01:29
So this pcr is used for once amplified, the dna product by pcr can be used in many different laboratory procedures.
01:40
For example, in mapping, mostly in mapping techniques in human genome project.
01:50
And also pcr is also valuable in number of laboratory and clinical techniques including dna fingerprinting and detection of bacteria or viruses and diagnosis of many genetic disorders.
02:17
So how does this pcr actually works? so this pcr to amplify the segment of dna using pcr, the sample is first heated.
02:30
So we have to first hate it.
02:32
So the dna, denature, so there will be a denaturing of dna will occur.
02:38
For example, at first, the dna will be in the double standard form.
02:43
So when we denature it, it will change this into a single standard or denatured with denatures or separates into purbis of single standard dna...
