Question

1. Describe, stepwise, how you would prepare 0.5 L of a 0.15 M phosphate buffer, pH 12.5, using K3PO4·H2O (f.w. = 230.28) and K2HPO4 (f.w. = 174.18). pH = pKa + log [A-] ? [HA] H3PO4 ? H2PO4- + H+ pKa1 = 2.12 H2PO4- ? HPO4 2- + H+ pKa2 = 6.8 HPO4 2- ? PO4 3- + H+ pKa3 = 12.32 2. What is the enzyme that catalyzes the reaction CO2 + H2O ? H2CO3 in human tissues, including blood? 3. Since this reaction takes place in the absence of the enzyme, what is the physiological advantage in having such enzyme in the blood? [Hint: find what the turnover number is for this enzyme.] 4. This enzyme is also found in chloroplasts. What do you think the role of this enzyme is in plants?

          1. Describe, stepwise, how you would prepare 0.5 L of a 0.15 M phosphate buffer, pH 12.5, using K3PO4·H2O (f.w. = 230.28) and K2HPO4 (f.w. = 174.18).

pH = pKa + log [A-] ? [HA]

H3PO4 ? H2PO4- + H+ pKa1 = 2.12
H2PO4- ? HPO4 2- + H+ pKa2 = 6.8
HPO4 2- ? PO4 3- + H+ pKa3 = 12.32

2. What is the enzyme that catalyzes the reaction CO2 + H2O ? H2CO3 in human tissues, including blood?

3. Since this reaction takes place in the absence of the enzyme, what is the physiological advantage in having such enzyme in the blood? [Hint: find what the turnover number is for this enzyme.]

4. This enzyme is also found in chloroplasts. What do you think the role of this enzyme is in plants?

1. Describe, stepwise, how you would prepare 0.5 L of a 0.15 M phosphate buffer, pH 12.5, using K3PO4·H2O (f.w. = 230.28) and K2HPO4 (f.w. = 174.18).

pH = pKa + log [A-] ? [HA]

H3PO4 ? H2PO4- + H+ pKa1 = 2.12
H2PO4- ? HPO4 2- + H+ pKa2 = 6.8
HPO4 2- ? PO4 3- + H+ pKa3 = 12.32

2. What is the enzyme that catalyzes the reaction CO2 + H2O ? H2CO3 in human tissues, including blood?

3. Since this reaction takes place in the absence of the enzyme, what is the physiological advantage in having such enzyme in the blood? [Hint: find what the turnover number is for this enzyme.]

4. This enzyme is also found in chloroplasts. What do you think the role of this enzyme is in plants?

Added by Jeffrey K.

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