Describe the wet mount method for fungi. 1. Sterile techniques 2. Excavate an edge colony, trying to minimize the amount of agar. 3. Transfer to a slide, washing off the colony with 70% ethanol. 4. Tease out the mycelium with a wire loop. 5. Add a drop of lactophenol cotton blue and cover with a coverslip. 6. Blot excess dye. 7. Microscopy. 8. Why is 70% ethanol used in the wet mount?
Added by Matthew S.
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**Sterile techniques**: Sterile techniques are essential to prevent contamination of the sample and to ensure accurate identification of the fungi. This includes using sterile tools, such as wire loops, pipettes, and slides, as well as working in a clean Show more…
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