Describe why, before Meselson and Stahl's experiment, the conservative mode of replication was favored.
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This idea was based on the assumption that the original DNA molecule would serve as a template for the synthesis of a new, identical molecule, without any changes to the original molecule itself. This mode of replication was considered more likely because it Show more…
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4. Explain how Meselson and Stahl proved that DNA replication was semi conservative, with reference to the diagram below? Explain the experiment? (3 marks) /3 Conservative Semi-conservative Dispersive CsCl ultracentrifugation
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Imagine the Meselson and Stahl experiments had supported conservative replication instead of semiconservative replication. What results would you predict to observe after two rounds of replication? Be specific regarding percent distributions of DNA incorporating $^{15} \mathrm{N}$ and $^{14} \mathrm{N}$ in the gradient.
Suppose that Meselson and Stahl had carried out their experiment by growing cells in medium with $^{14} \mathrm{N}$ and then transferring the cells to medium containing $^{15} \mathrm{N}$. How would the bands within the centrifuge tubes have appeared if replication were semiconservative? If replication were conservative? If replication were dispersive?
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