1) Describe in detail why the Gram stain procedures stain Gram + bacteria purple, and Gram – bacteria pink. DO NOT JUST SAY ‘THICK OR THINNER CELL WALL’. I want to know the detailed specifics of the steps that result in WHY this procedure stains cells differentially. Think and find out the mechanisms of how each of the procedures.
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**Crystal Violet Staining**: Both Gram-positive and Gram-negative bacteria are stained with crystal violet, which binds to the peptidoglycan layer in the cell wall. At this stage, both types of bacteria appear purple. Show more…
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How is Gram stain done and what types of bacteria are stained differently in the Gram stain?
Adi S.
need an explanation of how the gram stain is used and why it works. (this is not the gram stain procedure, but rather, how the dyes and steps work together to show Gram positive and Gram negative cell wall types)
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