00:01
Okay, so we have this gel.
00:06
We have the latter in lane one, then lane two, three, four, and then five.
00:16
So in lane one, or sorry, lane two, we have the uncut plasmid here, which corresponds to roughly just a little bit above the 3 ,700 base pair band.
00:33
So that makes the uncut p -adams plasmid as 3 ,800 base pairs.
00:41
Now, in the last lane, which is a double digest of bamh1 and pst1, you see two bands.
00:53
And based on where it falls on the latter, it turns out to be that one of them is 3 ,300 base pairs.
01:04
And the other one is roughly 500 base pairs.
01:09
So together, this means that the total length of this plasma is 3 ,800 base pairs.
01:18
So it looks like this uncut plasmid is linearized.
01:23
So this likely means that this is linear because of this.
01:37
Let me double check.
01:52
So if this is the case, if a double digest gives us a 3 ,300 base pair and a 500 base here, that means we're the single digest in lane 3 and lane 4, we really should just only get one band at 3 ,800 each...