determine the type of inhibition of an enzyme catalyzed reaction from the shown data collected in the presence and absence of the inhibitor
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There are three main types of enzyme inhibition: competitive, non-competitive, and uncompetitive. In competitive inhibition, the inhibitor binds to the active site of the enzyme, preventing the substrate from binding. In non-competitive inhibition, the inhibitor Show more…
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Determine the type of inhibition of an enzymatic reaction from the following data collected in the presence and absence of the inhibitor. $$\begin{array}{ccc} \hline & & v_{0} \text { with I present } \\ {[\mathrm{S}](\mathrm{mM})} & v_{0}\left(\mathrm{mM} \cdot \min ^{-1}\right) & \left(\mathrm{m} \mathrm{M} \cdot \min ^{-1}\right) \\ \hline 1 & 1.3 & 0.8 \\ \hline 2 & 2.0 & 1.2 \\ \hline 4 & 2.8 & 1.7 \\ \hline 8 & 3.6 & 2.2 \\ \hline 12 & 4.0 & 2.4 \\ \hline \end{array}$$
Given the kinetics data for each enzyme in the presence and absence of its inhibitor, determine the type of inhibition. Enzyme | Km (mM) | Vmax (mmol/s) carbonic anhydrase | 8,000 | 600,000 + inhibitor A | 12,000 | 600,000 chymotrypsin | 5,000 | 100 + inhibitor B | 5,000 | 75 penicillinase | 50 | 2,000 + inhibitor C | 30 | 1,500 lysozyme | 6 | 0.5 + inhibitor D | 15 | 0.5 carboxypeptidase A | 3 | 1,000 + inhibitor E | 3 | 800 Competitive Noncompetitive Uncompetitive Answer Bank inhibitor D, inhibitor B, inhibitor A, inhibitor E, inhibitor C
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Suppose that the data shown below are obtained for an enzyme-catalyzed reaction in the presence and absence of an inhibitor: V (mmol ml- min) Without inhibitor With inhibitor [SKmM) 5.0 20 3.0 4.0 43 0.8 100 10.7 12.5 13.6 40 5.5 Using double-reciprocal plots of the data, determine the type of inhibition that has occurred.
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