Question

DETERMINING HEAT CAPACITY CHANGES UPON PROTEIN UNFOLDING: DIFFERENTIAL SCANNING CALORIMETRY (DSC). (3 + 2 = 5 pts) a. Draw the complete DSC plot (Cp vs T) for the equilibrium unfolding of a protein that is sufficiently unstable so that both its cold- and heat-induced unfolding transitions can be observed in liquid solution by DSC. Be sure to draw lines, curves and areas with a biologically meaningful sign, based on what you know about protein folding and unfolding at equilibrium. Label Tm, Tm’, Cp,heat-unfolding and Cp,cold-unfolding on the plot. b. Draw the DSC profile for the order-disorder transition of a nonpolar lipid with Cp = 0.

          DETERMINING HEAT CAPACITY CHANGES UPON PROTEIN UNFOLDING:
DIFFERENTIAL SCANNING CALORIMETRY (DSC).
(3 + 2 = 5 pts)
a. Draw the complete DSC plot (Cp vs T) for the equilibrium unfolding of a protein that is
sufficiently unstable so that both its cold- and heat-induced unfolding transitions can be
observed in liquid solution by DSC. Be sure to draw lines, curves and areas with a biologically
meaningful sign, based on what you know about protein folding and unfolding at equilibrium.
Label Tm, Tm’, Cp,heat-unfolding and Cp,cold-unfolding on the plot.
b. Draw the DSC profile for the order-disorder transition of a nonpolar lipid with Cp = 0.

Added by Harper U.

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