DISCUSSION POST #1 Your post should include a drawing of...

DISCUSSION POST #1 Your post should include a drawing of the bacterial replication fork with all the enzymes in their proper locations. You must also describe the purpose of parts (b)-(k) in the same level of detail as this video described DNA polymerase III. 15. Diagram a replication fork in bacterial DNA and label the following structures or molecules. a. DNA pol III b. helicase, c. RNA primer d. origin of replication e. leading strand (label its polarity) f. DNA pol I g. topoisomerase h. SSB protein i. lagging strand (label its polarity) j. primase k. Okazaki fragment Explain purpose of parts (b-k).

Transcript

00:01 Hello students in this question we have to match appropriate labels to the description given below based on e.

00:10 Coli dna replication fork diagram provided in the question.

00:14 This is the diagram which has been provided in the question and just by looking at the diagram we should be able to make out which is the leading strand and the lagging strand before solving the question.

00:29 Here as we can see the newly synthesized dna is in fragments which are known as okazaki fragments therefore this is the lagging strand and this is the leading strand now let us match the description with the labels the first description mentions three prime end of the lagging strand here as i said earlier this is the lagging strand and this is the three prime end of the lagging strand therefore the answer to the question one is f.

01:04 The second description states that relieves the tension due to the helicase activity further down the dna.

01:12 This is the helicase and it is unwinding the double standard dna and this is the gyrase which is present downstream the dna the replication fork which helps in relieving the tension.

01:27 Therefore the answer to the second question is the next description states keeps single standard dna from forming hydrogen bonds with the complementary strand.

01:42 Here as we can see these are the single standard single strand binding proteins which are doing the particular function therefore the correct answer to this question is b.

01:55 The next description states eukaryotic dna polymerase delta in eukaryotes the so dna polymerization reaction is catalyzed by dna polymerase delta on the lagging side.

02:09 Therefore the correct label for the description is g.

02:16 The next descriptions are 5 prime end of the lagging strand and next one is 5 prime end of the okasaki fragment.

02:25 Since lagging strand and the okasaki fragments are one and the same, the label e is the 5 prime of the leading strand and the okasaki fragment.

02:39 The next description is helikaze...

Question

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