DNA polymerase I has 5'-3' polymerase activity, 5'-3' exonuclease activity, and 3'-5' exonucleas

DNA polymerase must possess 5'-3' polymerase activity, 5'-3' exonuclease

Question

DNA polymerase I has 5'-3' polymerase activity, 5'-3' exonuclease activity, and 3'-5' exonuclease activity necessary for DNA replication. Mutations in the gene that encodes DNA polymerase I may cause the enzyme to lose these activities. Match the consequence of a loss-of-function mutation in DNA polymerase I to the corresponding lost activity. Lost 5'-3' polymerase activity Lost 5'-3' exonuclease activity Lost 3'-5' exonuclease activity Decreased polymerase fidelity No DNA synthesis to fill gaps caused by removing RNA primers No RNA primer removal during DNA replication No double helix denaturation Unstable strand separation within the replication bubble Incorrect. Whereas other consequences of DNA polymerase I mutations may be mismatched, lost ability to synthesize DNA to fill gaps has not been correctly placed. Gaps are created when an enzyme removes the RNA primer that was necessary to initiate synthesis of a growing DNA strand. DNA polymerase I both removes the RNA primer and replaces the RNA bases with DNA. If DNA synthesis occurs in the 5'-3' direction, what activity must DNA polymerase possess to replace an RNA primer with DNA nucleotides?

          DNA polymerase I has 5'-3' polymerase activity, 5'-3' exonuclease activity, and 3'-5' exonuclease activity necessary for DNA replication. Mutations in the gene that encodes DNA polymerase I may cause the enzyme to lose these activities.

Match the consequence of a loss-of-function mutation in DNA polymerase I to the corresponding lost activity.

Lost 5'-3' polymerase activity
Lost 5'-3' exonuclease activity
Lost 3'-5' exonuclease activity

Decreased polymerase fidelity
No DNA synthesis to fill gaps caused by removing RNA primers
No RNA primer removal during DNA replication

No double helix denaturation
Unstable strand separation within the replication bubble

Incorrect.
Whereas other consequences of DNA polymerase I mutations may be mismatched, lost ability to synthesize DNA to fill gaps has not been correctly placed. Gaps are created when an enzyme removes the RNA primer that was necessary to initiate synthesis of a growing DNA strand. DNA polymerase I both removes the RNA primer and replaces the RNA bases with DNA.

If DNA synthesis occurs in the 5'-3' direction, what activity must DNA polymerase possess to replace an RNA primer with DNA nucleotides?

DNA polymerase I has 5'-3' polymerase activity, 5'-3' exonuclease activity, and 3'-5' exonuclease activity necessary for DNA replication. Mutations in the gene that encodes DNA polymerase I may cause the enzyme to lose these activities.

Match the consequence of a loss-of-function mutation in DNA polymerase I to the corresponding lost activity.

Lost 5'-3' polymerase activity
Lost 5'-3' exonuclease activity
Lost 3'-5' exonuclease activity

Decreased polymerase fidelity
No DNA synthesis to fill gaps caused by removing RNA primers
No RNA primer removal during DNA replication

No double helix denaturation
Unstable strand separation within the replication bubble

Incorrect.
Whereas other consequences of DNA polymerase I mutations may be mismatched, lost ability to synthesize DNA to fill gaps has not been correctly placed. Gaps are created when an enzyme removes the RNA primer that was necessary to initiate synthesis of a growing DNA strand. DNA polymerase I both removes the RNA primer and replaces the RNA bases with DNA.

If DNA synthesis occurs in the 5'-3' direction, what activity must DNA polymerase possess to replace an RNA primer with DNA nucleotides?

Added by Martin G.

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