do you expect the results of the luciferase and molybdate assays to reveal the same patter of atp synthesis?
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Adenosine triphosphate (ATP) is the molecule used for energy storage by all types of living cells (animal, plant, bacterial, yeast, and mold). ATP transfers energy within living cells to power the enzymes needed for cellular functions. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery. Various ATP assays have been developed to rapidly and reliably quantify intracellular ATP. A reliable assay shows that the ATP (adenosine triphosphate) content of a certain type of cell is 111 ÎĽmol/100mL. You developed a new assay, which gave the values 117, 119, 111, 115, 120 ÎĽmol/100mL for replicate analyses. Do your results agree with the known value at the 95% confidence level? What do these results tell you about your new method?
Adi S.
If you had used a DNA lysate containing viable cells, would it have been possible to determine whether transformation had taken place? Explain.
what will you observe if the inhibitors of ATP synthase also lead to an inhibition of the electron-transport chain?
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