12. Draw the expected RFLP results for the three individuals...

12. Draw the expected RFLP results for the three individuals shown (1-3) on the gel. Enter the genotypes of each person below the gel (e.g. +/+ if the internal cut site correlates to a dominant genotype (+). 700 300 1000 900 800 700 600 500 400 300 200 100 a. 13. A 100 bp VNTR exists at a locus in the human genome. Two parents have four children with different genotypes at this locus. Draw the bands you would expect to see on a gel for both the parents and all four children, and the genotype below each (e.g. 3/4). 100 bp repeat Mother Father 1000 900 800 700 600 500 400 300 200 100 a. 1000 900 800 700 600 500 400 300 200 100 b. The parents have a fifth child, with genotype 5/5. What are some possible explanations for this?

Transcript

00:01 So question number two is a rflp results question that corresponding to gel atrophoresis.

00:09 So there are three individuals, and each individual has two alleles.

00:13 And then on these two alleles, there are different restriction sites.

00:16 Let's take a look at individual number one.

00:19 So for individual number one, there are two alleles.

00:22 Both have three restriction sites, and i use arrow to show where the sites are.

00:28 So after cutting by the restriction enzyme, both alleles produce two fragments.

00:35 One is between the first and second restriction site, and this produced 700 base pair band.

00:43 And from the second restriction site into the third restriction site, it produced a 300 base pair band.

00:50 So that's the same for the bottom allele.

00:54 Again, it is cut three times and produced.

00:58 700 base pair and 300 base pair men.

01:02 So now for individual one you actually will end up having two fragments.

01:06 One is 700, the other is 300 base pair.

01:09 So let's take a look at this gel on the right side.

01:12 So the tallest, the top band is 1 ,000.

01:16 So this is 900, 800, and 700.

01:23 So then one restriction fragment will show up at 700 base pair and then the other is 300.

01:29 300 is right here.

01:35 So you can see for individual number one after restriction rflp this individual will produce two different restriction fragments, 700 and 300.

01:47 Now let's take a look at individual number two.

01:51 So number two also has two alleles.

01:55 For the top allios there are three restrictions site.

01:59 So for the top allio after restriction site, after restriction enzyme digestion, it will produce a 700 base pair band and 300 base pair band.

02:11 The bottom allele does not have the middle restriction site.

02:17 That means the bottom allele is only being cut twice.

02:21 So it produced a longer restriction fragment, which is 700 plus 300.

02:27 So just end up 1 ,000.

02:30 So now, if you take a look at all the restriction fragments produced from individual number one, you actually see three different bands.

02:41 One is at 700, and then the other is 300, and the last one is 1 ,000, which is from the bottom allele.

02:56 And this will run at the 1 ,000 base pair position on top of the gel.

03:03 Now let's take a look at individual number three.

03:07 So again, for number three, this person also has two alleles.

03:15 But for both alleles, there are only two restrictions at the beginning and the end.

03:20 So this means that both alleles will produce 700 plus 300, which is 1 ,000.

03:30 This is for top alleles, the bottom, same thing, only two restrictions site.

03:34 So 1 ,000 from the bottom allele.

03:38 So that means both top and bottom allele will give you 1 ,000 base pair restriction fragment.

03:43 And this will run at the top where the band matches 1 ,000 base pair.

03:51 So you can see that individual number one produced two restriction bands, 700 and 300 base pair.

03:59 And for individual number two, that person produced three restriction fragments, 1 ,700, and 300.

04:09 And for individual number three, only produce one restriction fragment.

04:14 Both fragments runs at 1 ,000 base pair position.

04:17 So this is question number 12.

04:21 Let's move on to 13.

04:23 So for 13, it's a vntr question.

04:26 So it's a short tandem repeat, which is 100 base pair.

04:31 Pair.

04:31 So there are two parents.

04:33 They have four children with different genotypes at this locus.

04:37 So the question asks you to draw the bands you would expect to see on a gel for both parents and all four children...

Question

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