00:01
The microbial straining which can be given as ms is the staining techniques that is used to enhance the contrast at microscopic level and it is used to stain microorganisms which is given as m -woaks.
00:21
These microorganisms are stained when they are alive or sometimes even when they are dead.
00:32
Differential staining differential staining is a staining technique that is a staining technique that is a use as multiple stains or it is simply usage of more than one stain to differentiate the structures or differentiate various cellular organisms or cellular structures which are present in the same slide.
00:57
The example of the differential stain is the gram stain.
01:03
The gram stain contains two stains, namely the crystal violet.
01:10
That is used to stain gram positive bacteria and other stain is called as the counterstain saffranin that stains the gram negative bacteria.
01:31
Let us see the preparation of a bacterial smear.
01:36
A bacterial smear is simply a slide that contains the bacterial culture.
01:42
The first step will be taking a clean slide and wiping it off with alcohol.
01:48
Alcohol.
01:50
This is to disinfect them and after wiping off with alcohol we have to dry the slide.
01:59
Then an inoplation loop is taken and it is made sure that the inoppelation loop is sterile in condition and it is used to hold the culture media that contains the organism some that has to be smeared in the slide and with the help of this inoculation loop, the culture is spread all over the slide.
02:34
So if this is the slide, the culture is spread so that it is present all over the slide.
02:45
The next step after spreading is the fixation.
02:50
It is air dried and the slide is heat fixed...