Effect of Enzyme Concentration on Reaction Rate Reaction rate (1/sec) 0.0200 0.0160 0.0120 0.0080 0.0040 0.0000 0.00 0.10 0.20 0.30 0.40 0.50 Amylase concentration (%) y = -0.104x² + 0.0834x + 0.0009 R² = 0.9756 Discussion 1. Explain your results in terms of your hypothesis. Describe the shape of the reaction rate curve as enzyme concentration increases. What factors are responsible for the shape of this curve? (3 points) According to the graph the curve represent the 2. Speculate about the shape of a curve measuring reaction rate if you had increased the concentration of enzyme, but held the concentration of substrate constant. (3 points)
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As the enzyme concentration increases, the reaction rate also increases. This is because there are more enzyme molecules available to catalyze the reaction, leading to more product formation in a given time. Show more…
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C. Study of the effect of enzyme concentration on the rate of the reaction 1. Record your observations in the following table. Tube # | Enzyme Concentration | Height of Bubbles (cm) 1 | 100 % 2 | 50 % 3 | 25 % 4 | 12.5 % 2. Plot a graph of your observed data. 3. From the above graph, what can you conclude about the rate of a chemical reaction when concentration of the enzyme is decreased?
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The graph below shows the effect of substrate concentration on enzyme activity. What conclusion can be drawn about section X of the graph? Enzyme activity Substrate concentration The enzyme has started to denature and the reaction slows down. The enzyme is saturated and is working at its maximum reaction rate. Some of the enzyme has been consumed and the reaction has reached a plateau. The reaction has finished and the substrate has been used up.
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