endoglycase H followed by o-glyconase. The numbers at the bottom of the gel image refer to the lane numbers. The positions of molecular weight markers are shown on the left. The plus and minus symbols above the gel image refer to whether the enzymes were present or not, before running the polyacrylamide gel electrophoresis. Lane 1 was untreated protein samples revealing 2 proteins of 76 and 36 kDa. Those proteins were then treated with Engoglycase H (lane 2) or with Endoglycase H followed by 0-glyconase (lane 3). Based on these results, which of the following statements is not true? A. The 76 KDa protein has an N-linked sugar but not and 0-linked sugar. B. The 36Kda protein has both N-linked and O-linked sugars C. The 36 kDa protein shows a reduction in its molecular weight following digestion with both Endo-H and O-glyconase D. The 76 KDa protein has both N-linked and O-linked sugars E. All are true
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This means that the 76 kDa protein has both N-linked and O-linked sugars. Show more…
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