00:01
Okay, so in this question, it's basically saying this is schematic that this organism uses.
00:09
So this is a schematic outlining a metabolic pathway that this bacteria uses, or a metabolic pathway that an organism uses.
00:19
Let's just go with bacteria because it's easiest.
00:22
Basically, metabolite a is converted to metabolite b through enzyme.
00:31
Enzyme number one.
00:38
And then b is then converted to c with enzyme two.
00:44
So the question is this.
00:46
If there is a bacteria in which there is a mutant in enzyme one, there's a mutant in that enzyme one no longer exists, meaning that a can no longer be converted to b, under which conditions can this bacteria grow since enzyme a is, or enzyme one is no longer functional? so the answer here is, oh, and one more thing i should mention, all three of this, and a, b, actually, let's do it this way, a, b, and c are all substances required for growth.
01:49
So, and, but it's required because, again, remember, enzyme a, or compound a is converted to b because of enzyme one.
01:59
So if that's the case, under which conditions can they grow? ultimately, what is necessary is this right here, compound c, that's the end of the metabolic pathway.
02:12
So ultimately, we need to use a supplemented media.
02:22
So two of the answers, minimal media or minimal media with no supplements, is essentially the same thing, they're both still minimal media.
02:30
Minimal media with nothing at it is still minimal media...