Enzymes bind to substrates with a specific affinity, called Question Blank 1 of 20. For example, hexokinase binds to ATP (Km = 0.4 mM) and glucose (Km = 50 M), and thus it binds to Question Blank 2 of 20 at a lower concentration and much more tightly. Catalyzed reactions are dependent upon substrate concentration to a point, and the maximum rate of catalysis where substrate concentrations only have a minimal effect is called Question Blank 3 of 20. Both Km and Vmax can be affected by inhibitors.