Eukaryotic gene regulation You isolate yeast chromatin and break it down to fragments with one or two nucleosomes. Using immunological (antibodies, no need to know any detail), you selectively isolate nucleosomes that have acetylated histone tails. You extract DNA from this purified fraction and sequence it. Comparing the sequence you obtain to the annotated genome sequence you expect to find the location of: A) active promoters B) crossing overs C) inactive promoters D) all nucleosomes E) transposons
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Step 1: Acetylation of histone tails neutralizes the positive charge of lysine residues, leading to a looser chromatin structure. Show more…
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Gene regulation in eukaryotes has some similarities to that in prokaryotes, but is quite distinct in the variety of mechanisms employed and their complexity. A. Name three differences between gene regulation in prokaryotes and eukaryotes. B. How is the preparation for transcription different in eukaryotes compared with prokaryotes? C. What is an enhancer? Explain its use in transcriptional control. Be certain to define basal level of transcription. D. Eukaryotic repression can work by several different mechanisms including competition and quenching. Explain briefly each type.
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One difference between prokaryotic and eukaryotic gene transcription is the need to open up the chromatin structure to enable access to the promoter and regulatory region. Which factors contribute to opening up the chromatin in eukaryotes? a. Histone methyltransferases and chromatin remodeling factors b. Histone methyltransferases and RNA polymerase III c. Histone acetyltransferases and chromatin remodeling factors. d. Histone acetyltransferases and DNA polymerase e. Only chromatin remodeling factors
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Which of the following is characteristic of genes and gene regulation in both bacteria and eukaryotes? (a) promoters (b) non-coding DNA within coding sequences (c) enhancers (d) operons (e) DNA located in a nucleus
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